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Hypothalamic–pituitary–adrenal axis


Hypothalamic–pituitary–adrenal axis

Basic HPA axis summary (corticotropin-releasing hormone=CRH, adrenocorticotropic hormone=ACTH).

The hypothalamic–pituitary–adrenal axis (HPA or HTPA axis), also known as the limbic–hypothalamic–pituitary–adrenal axis (LHPA axis) and, occasionally, as the hypothalamic–pituitary–adrenal–gonadotropic axis, is a complex set of direct influences and feedback interactions among three endocrine glands: the hypothalamus, the pituitary gland (a pea-shaped structure located below the hypothalamus), and the adrenal (also called "suprarenal") glands (small, conical organs on top of the kidneys).

The interactions among these organs constitute the HPA axis, a major part of the neuroendocrine system that controls reactions to stress and regulates many body processes, including digestion, the immune system, mood and emotions, sexuality, and energy storage and expenditure. It is the common mechanism for interactions among glands, hormones, and parts of the midbrain that mediate the general adaptation syndrome (GAS).[1] While steroid hormones are produced mainly in vertebrates, the physiological role of the HPA axis and corticosteroids in stress response is so fundamental that analogous systems can be found in invertebrates and monocellular organisms as well.


  • Anatomy 1
  • Function 2
  • Immune system 3
  • Stress 4
    • Stress and disease 4.1
    • Stress and development 4.2
      • Prenatal stress 4.2.1
      • Early life stress 4.2.2
  • See also 5
  • References 6
  • External links 7


The key elements of the HPA axis are:

CRH and vasopressin are released from neurosecretory nerve terminals at the median eminence. CRH is transported to the anterior pituitary through the portal blood vessel system of the hypophyseal stalk and vasopressin is transported by axonal transport to the posterior pituitary. There, CRH and vasopressin act synergistically to stimulate the secretion of stored ACTH from corticotrope cells. ACTH is transported by the blood to the adrenal cortex of the adrenal gland, where it rapidly stimulates biosynthesis of corticosteroids such as cortisol from cholesterol. Cortisol is a major stress hormone and has effects on many tissues in the body, including the brain. In the brain, cortisol acts on two types of receptor – mineralocorticoid receptors and glucocorticoid receptors, and these are expressed by many different types of neurons. One important target of glucocorticoids is the hypothalamus, which is a major controlling centre of the HPA axis.

Vasopressin can be thought of as "water conservation hormone" and is also known as "antidiuretic hormone." It is released when the body is dehydrated and has potent water-conserving effects on the kidney. It is also a potent vasoconstrictor.

Important to the function of the HPA axis are some of the feedback loops:

  • Cortisol produced in the adrenal cortex will negatively feedback to inhibit both the hypothalamus and the pituitary gland. This reduces the secretion of CRH and vasopressin, and also directly reduces the cleavage of proopiomelanocortin (POMC) into ACTH and β-endorphins.
  • Epinephrine and norepinephrine are produced by the adrenal medulla through sympathetic stimulation and the local effects of cortisol (upregulation enzymes to make E/NE). E/NE will positively feedback to the pituitary and increase the breakdown of POMCs into ACTH and β-endorphins.


Release of CRH from the hypothalamus is influenced by

  • Media related to at Wikimedia Commons
  • page on HPA axis
  • HPA Axis: Explanation of the Body's Central Stress Response System w/Diagram

External links

  1. ^ Selye, Hans (1974). Stress without distress. Philadelphia: Lippincott.  
  2. ^ a b c d editors, volume; Besedovsky, Hugo; Chrousos, George; Rey, Adriana Del (2008). The hypothalamus-pituitary-adrenal axis (1st ed.). Amsterdam: Academic.  
  3. ^ MacHale SM, Cavanagh JT, Bennie J, Carroll S, Goodwin GM, Lawrie SM (November 1998). "Diurnal variation of adrenocortical activity in chronic fatigue syndrome". Neuropsychobiology 38 (4): 213–7.  
  4. ^ Backhaus J, Junghanns K, Hohagen F (October 2004). "Sleep disturbances are correlated with decreased morning awakening salivary cortisol". Psychoneuroendocrinology 29 (9): 1184–91.  
  5. ^ Pruessner JC, Hellhammer DH, Kirschbaum C (1999). "Burnout, perceived stress, and cortisol responses to awakening". Psychosom Med 61 (2): 197–204.  
  6. ^ Spencer RL, Hutchison KE (1999). "Alcohol, aging, and the stress response". Alcohol Research & Health 23 (4): 272–83.  
  7. ^ Pariante CM (August 2003). "Depression, stress and the adrenal axis". Journal of Neuroendocrinology 15 (8): 811–2.  
  8. ^ Douglas AJ (March 2005). "Central noradrenergic mechanisms underlying acute stress responses of the Hypothalamo–pituitary–adrenal axis: adaptations through pregnancy and lactation". Stress 8 (1): 5–18.  
  9. ^ Engelmann M, Landgraf R, Wotjak CT (2004). "The hypothalamic-neurohypophysial system regulates the hypothalamic–pituitary–adrenal axis under stress: an old concept revisited". Frontiers in Neuroendocrinology 25 (3–4): 132–49.  
  10. ^ Detillion CE, Craft TK, Glasper ER, Prendergast BJ, DeVries AC (September 2004). "Social facilitation of wound healing". Psychoneuroendocrinology 29 (8): 1004–11.  
  11. ^ Winberg S, Øverli Ø, Lepage O (November 2001). "Suppression of aggression in rainbow trout (Oncorhynchus mykiss) by dietary L-tryptophan". The Journal of Experimental Biology 204 (Pt 22): 3867–76.  
  12. ^ Coplan JD, Mathew SJ, Smith EL, et al. (July 2001). "Effects of LY354740, a novel glutamatergic metabotropic agonist, on nonhuman primate hypothalamic–pituitary–adrenal axis and noradrenergic function". CNS Spectrums 6 (7): 607–12, 617.  
  13. ^ Miller GE, Chen E, Zhou ES (January 2007). "If it goes up, must it come down? Chronic stress and the hypothalamic–pituitary–adrenocortical axis in humans". Psychological Bulletin 133 (1): 25–45.  
  14. ^ Kim JE, Cho BK, Cho DH, Park HJ (July 2013). "Expression of hypothalamic–pituitary–adrenal axis in common skin diseases: evidence of its association with stress-related disease activity". Acta Dermato-venereologica 93 (4): 387–93.  
  15. ^ Koehl M, Darnaudéry M, Dulluc J, Van Reeth O, Le Moal M, Maccari S (September 1999). "Prenatal stress alters circadian activity of hypothalamo–pituitary–adrenal axis and hippocampal corticosteroid receptors in adult rats of both gender". Journal of Neurobiology 40 (3): 302–15.  
  16. ^ Weinstock M, Matlina E, Maor GI, Rosen H, McEwen BS (November 1992). "Prenatal stress selectively alters the reactivity of the hypothalamic-pituitary adrenal system in the female rat". Brain Research 595 (2): 195–200.  
  17. ^ Weinstock M (August 2008). "The long-term behavioural consequences of prenatal stress" (PDF). Neuroscience and Biobehavioral Reviews 32 (6): 1073–86.  
  18. ^ Gutteling BM, de Weerth C, Buitelaar JK (December 2004). "Maternal prenatal stress and 4-6 year old children's salivary cortisol concentrations pre- and post-vaccination". Stress 7 (4): 257–60.  
  19. ^ Buitelaar JK, Huizink AC, Mulder EJ, de Medina PG, Visser GH (2003). "Prenatal stress and cognitive development and temperament in infants". Neurobiology of Aging. 24 Suppl 1: S53–60; discussion S67–8.  
  20. ^ a b Flinn MV, Nepomnaschy PA, Muehlenbein MP, Ponzi D (June 2011). "Evolutionary functions of early social modulation of hypothalamic–pituitary–adrenal axis development in humans". Neuroscience and Biobehavioral Reviews 35 (7): 1611–29.  
  21. ^ Liu D, Diorio J, Tannenbaum B, et al. (September 1997). "Maternal care, hippocampal glucocorticoid receptors, and hypothalamic–pituitary–adrenal responses to stress". Science 277 (5332): 1659–62.  
  22. ^ Macrì S, Würbel H (December 2006). "Developmental plasticity of HPA and fear responses in rats: a critical review of the maternal mediation hypothesis". Hormones and Behavior 50 (5): 667–80.  
  23. ^ de Kloet ER, Sibug RM, Helmerhorst FM, Schmidt MV, Schmidt M (April 2005). "Stress, genes and the mechanism of programming the brain for later life". Neuroscience and Biobehavioral Reviews 29 (2): 271–81.  
  24. ^ Schechter DS, Moser DA, Paoloni-Giacobino A, Stenz A, Gex-Fabry M, Aue T, Adouan W, Cordero MI, Suardi F, Manini A, Sancho Rossignol A, Merminod G, Ansermet F, Dayer AG, Rusconi Serpa S (epub May 29, 2015). Methylation of NR3C1 is related to maternal PTSD, parenting stress and maternal medial prefrontal cortical activity in response to child separation among mothers with histories of violence exposure. Frontiers in Psychology. To view the online publication, please click here:
  25. ^ Heim C., Newport D. J., Heit S., Graham Y. P., Wilcox M., Bonsall R., Nemeroff C. B. (2000). "Pituitary-adrenal and autonomic responses to stress in women after sexual and physical abuse in childhood". JAMA 284 (5): 592–597.  
  26. ^ a b c Heim C., Newport D.J., Bonsall R., Miller A.H., Nemeroff C.B. (2001). "Altered Pituitary-Adrenal Axis Responses to Provocative Challenge Tests in Adult Survivors of Childhood Abuse". Am J Psychiatry 158 (4): 575–581.  
  27. ^ Denver RJ (Apr 2009). "Structural and functional evolution of vertebrate neuroendocrine stress systems". Ann N Y Acad Sci. 1163: 1–16.  
  28. ^ a b Oitzl MS, Champagne DL, van der Veen R, de Kloet ER (May 2010). "Brain development under stress: hypotheses of glucocorticoid actions revisited". Neurosci Biobehav Rev 34 (6): 853–66.  
  29. ^ a b Horton TH (Jan 2005). "Fetal origins of developmental plasticity: animal models of induced life history variation". Am J Hum Biol. 17 (1): 34–43.  
  30. ^ "Antenatal glucocorticoids and programming of the developing CNS.". Pediatr Res 47: 291–300. Mar 2000.  
  31. ^ a b "Variations in maternal care in the rat as a mediating influence for the effects of environment on development.". Physiol Behav 79: 359–71. Aug 2003.  
  32. ^ a b c Daskalakis NP, Bagot RC, Parker KJ, Vinkers CH, de Kloet ER (Sep 2013). "The three-hit concept of vulnerability and resilience: toward understanding adaptation to early-life adversity outcome". Psychoneuroendocrinology 38 (9): 1858–73.  
  33. ^ a b Roth TL, Matt S, Chen K, Blaze J (Dec 2014). "Bdnf DNA methylation modifications in the hippocampus and amygdala of male and female rats exposed to different caregiving environments outside the homecage". Dev Psychobiol 56 (8): 1755–63.  



See also

Ultimately, the conservation of the HPA axis has underscored its critical adaptive roles in vertebrates, so, too, various invertebrate species over time. The HPA Axis plays a clear role in the production of corticosteroids, which govern many facets of brain development and responses to ongoing environmental stress. With these findings, animal model research has served to identify what these roles are – with regards to animal development and evolutionary adaptation. In more precarious, primitive times, a heightened HPA axis may have served to protect organisms from predators and extreme environmental conditions, such as weather and natural disasters, by encouraging migration (i.e. fleeing), the mobilization of energy, learning (in the face of novel, dangerous stimuli) as well as increased appetite for biochemical energy storage. In contemporary society, the endurance of the HPA axis and early life programming will have important implications for counseling expecting and new mothers, as well as individuals who may have experienced significant early life adversity.[33]

(2) Three-Hit Concept of vulnerability and resilience:[32] this hypothesis states that within a specific life context, vulnerability may be enhanced with chronic failure to cope with ongoing adversity. It fundamentally seeks to explicate why, under seemingly indistinguishable circumstances, one individual may cope resiliently with stress, whereas another may not only cope poorly, but consequently develop a stress-related mental illness. The three “hits” – chronological and synergistic – are as follows: genetic predisposition (which predispose higher/lower HPA axis reactivity), early-life environment (perinatal – i.e. maternal stress, and postnatal – i.e. maternal care), and later-life environment (which determines match/mismatch, as well as a window for neuroplastic changes in early programming).[33] (Figure 1)6 The concept of match/mismatch is central to this evolutionary hypothesis. In this context, it elucidates why early life programming in the perinatal and postnatal period may have been evolutionarily selected for. Specifically, by instating specific patterns of HPA axis activation, the individual may be more well equipped to cope with adversity in a high-stress environment. Conversely, if an individual is exposed to significant early life adversity, heightened HPA axis reactivity may “mismatch” him/her in an environment characterized by low stress. The latter scenario may represent maladaptation due to early programming, genetic predisposition, and mismatch. This mismatch may then predict negative developmental outcomes such as psychopathologies in later life.

(1) The predictive adaptation hypothesis:[32] this hypothesis is in direct contrast with the diathesis stress model, which posits that the accumulation of stressors across a lifespan can enhance the development of psychopathology once a threshold is crossed. Predictive adaptation asserts that early life experience induces epigenetic change; these changes predict or “set the stage” for adaptive responses that will be required in his/her environment. Thus, if a developing child (i.e., fetus to neonate) is exposed to ongoing maternal stress and low levels of maternal care (i.e., early life adversity), this will program his/her HPA axis to be more reactive to stress. This programming will have predicted, and potentially be adaptive in a highly stressful, precarious environment during childhood and later life. The predictability of these epigenetic changes is not definitive, however – depending primarily on the degree to which the individual's genetic and epigenetically modulated phenotype “matches” or “mismatches” with his/her environment (See: Hypothesis (2)).

Various hypotheses have been proposed, in attempts to explain why early life adversity can produce outcomes ranging from extreme vulnerability to resilience, in the face of later stress. Glucocorticoids produced by the HPA axis have been proposed to confer either a protective or harmful role, depending on an individual's genetic predispositions, programming effects of early-life environment, and match or mismatch with one's postnatal environment. The predictive adaptation hypothesis (1), the three-hit concept of vulnerability and resilience (2) and the maternal mediation hypothesis (3) attempt to elucidate how early life adversity can differentially predict vulnerability or resilience in the face of significant stress in later life.[32] These hypotheses are not mutually exclusive but rather are highly interrelated and unique to the individual.

The HPA Axis was present in the earliest vertebrate species, and has remained highly conserved by strong positive selection due to its critical adaptive roles.[27] The programming of the HPA axis is strongly influenced by the perinatal and early juvenile environment, or “early-life environment.” [28][29][30] Maternal stress and differential degrees of caregiving may constitute early life adversity, which has been shown to profoundly influence, if not permanently alter, the offspring's stress and emotional regulating systems.[28][29] Widely studied in animal models (e.g. licking and grooming/LG in rat pups),[31] the consistency of maternal care has been shown to have a powerful influence on the offspring's neurobiology, physiology, and behavior. Whereas maternal care improves cardiac response, sleep/wake rhythm, and growth hormone secretion in the neonate, it also suppresses HPA axis activity. In this manner, maternal care negatively regulates stress response in the neonate,[31] thereby shaping his/her susceptibility to stress in later life. These programming effects are not deterministic, as the environment in which the individual develops can either match or mismatch with the former's “programmed” and genetically predisposed HPA axis reactivity. Although the primary mediators of the HPA axis are known, the exact mechanism by which its programming can be modulated during early life remains to be elucidated. Furthermore, evolutionary biologists contest the exact adaptive value of such programming, i.e. whether heightened HPA axis reactivity may confer greater evolutionary fitness.

Heim and colleagues have proposed that early life stress, such as childhood abuse, can induce a sensitization of the HPA axis, resulting in particular heightened neuronal activity in response to stress-induced CRF release.[26] With repeated exposure to stress, the sensitized HPA axis may continue to hypersecrete CRF from the hypothalamus. Over time, CRF receptors in the anterior pituitary will become down-regulated, producing depression and anxiety symptoms.[26] This research in human subjects is consistent with the animal literature discussed above.

Though animal models allow for more control of experimental manipulation, the effects of early life stress on HPA axis function in humans has also been studied. One population that is often studied in this type of research is adult victims of childhood abuse. Adult victims of childhood abuse have exhibited increased ACTH concentrations in response to a psychosocial stress task compared to healthy controls and subjects with depression but not childhood abuse.[25] In one study, adult victims of childhood abuse that are not depressed show increased ACTH response to both exogenous CRF and normal cortisol release. Adult victims of childhood abuse that are depressed show a blunted ACTH response to exoegenous CRH.[26] A blunted ACTH response is common in depression, so the authors of this work posit that this pattern is likely to be due to the participant's depression and not their exposure to early life stress.

Another possibility is that the effects of early-life stress on HPA functioning are mediated by maternal care. Frequent human handling of the rat pups may cause their mother to exhibit more nurturant behavior, such as licking and grooming. Nurturant maternal care, in turn, may enhance HPA functioning in at least two ways. First, maternal care is crucial in maintaining the normal stress hypo responsive period (SHRP), which in rodents, is the first two weeks of life during which the HPA axis is generally non-reactive to stress. Maintenance of the SHRP period may be critical for HPA development, and the extreme stress of maternal separation, which disrupts the SHRP, may lead to permanent HPA dysregulation.[23] Another way that maternal care might influence HPA regulation is by causing epigenetic changes in the offspring. For example, increased maternal licking and grooming has been shown to alter expression of the glutocorticoid receptor gene implicated in adaptive stress response.[20] At least one human study has identified maternal neural activity patterns in response to video stimuli of mother-infant separation as being associated with decreased glucocorticoid receptor gene methylation in the context of post-traumatic stress disorder stemming from early life stress. [24] Yet clearly, more research is needed to determine if the results seen in cross-generational animal models can be extended to humans.

Several mechanisms have been proposed to explain these findings in rat models of early-life stress exposure. There may be a critical period during development during which the level of stress hormones in the bloodstream contribute to the permanent calibration of the HPA Axis. One experiment has shown that, even in the absence of any environmental stressors, early-life exposure to moderate levels of corticosterone was associated with stress resilience in adult rats, whereas exposure to high doses was associated with stress vulnerability.[22]

The role of early life stress in programming the HPA Axis has been well-studied in animal models. Exposure to mild or moderate stressors early in life has been shown to enhance HPA regulation and promote a lifelong resilience to stress. In contrast, early-life exposure to extreme or prolonged stress can induce a hyper-reactive HPA Axis and may contribute to lifelong vulnerability to stress.[20] In one widely-replicated experiment, rats subjected to the moderate stress of frequent human handling during the first two weeks of life had reduced hormonal and behavioral HPA-mediated stress responses as adults, whereas rats subjected to the extreme stress of prolonged periods of maternal separation showed heightened physiological and behavioral stress responses as adults.[21]

Early life stress

There is growing evidence that prenatal stress can affect HPA regulation in humans. Children who were stressed prenatally may show altered cortisol rhythms. For example, several studies have found an association between maternal depression during pregnancy and childhood cortisol levels.[18] Prenatal stress has also been implicated in a tendency toward depression and short attention span in childhood.[19] There is no clear indication that HPA dysregulation caused by prenatal stress can alter adult behavior.

There is evidence that prenatal stress can influence HPA regulation. In animal experiments, exposure to prenatal stress has been shown to cause a hyper-reactive HPA stress response. Rats that have been prenatally stressed have elevated basal levels and abnormal circadian rhythm of corticosterone as adults.[15] Additionally, they require a longer time for their stress hormone levels to return to baseline following exposure to both acute and prolonged stressors. Prenatally stressed animals also show abnormally high blood glucose levels and have fewer glucocorticoid receptors in the hippocampus.[16] In humans, prolonged maternal stress during gestation is associated with mild impairment of intellectual activity and language development in their children, and with behaviour disorders such as attention deficits, schizophrenia, anxiety and depression; self-reported maternal stress is associated with a higher irritability, emotional and attentional problems.[17]

Prenatal stress

Stress and development

It is also known that HPA axis hormones are related to certain skin diseases and skin homeostasis. There is evidence shown that the HPA axis hormones can be linked to certain stress related skin diseases and skin tumors. This happens when HPA axis hormones become hyperactive in the brain.[14]

Studies on people show that the HPA axis is activated in different ways during chronic stress depending on the type of stressor, the person's response to the stressor and other factors. Stressors that are uncontrollable, threaten physical integrity, or involve trauma tend to have a high, flat diurnal profile of cortisol release (with lower-than-normal levels of cortisol in the morning and higher-than-normal levels in the evening) resulting in a high overall level of daily cortisol release. On the other hand, controllable stressors tend to produce higher-than-normal morning cortisol. Stress hormone release tends to decline gradually after a stressor occurs. In post-traumatic stress disorder there appears to be lower-than-normal cortisol release, and it is thought that a blunted hormonal response to stress may predispose a person to develop PTSD.[13]

The HPA axis is a feature of mammals and other vertebrates. For example, biologists studying stress in fish showed that social subordination leads to chronic stress, related to reduced aggressive interactions, to lack of control, and to the constant threat imposed by dominant fish. Serotonin (5HT) appeared to be the active neurotransmitter involved in mediating stress responses, and increases in serotonin are related to increased plasma α-MSH levels, which causes skin darkening (a social signal in salmonoid fish), activation of the HPA axis, and inhibition of aggression. Inclusion of the amino acid L-tryptophan, a precursor of 5HT, in the feed of rainbow trout made the trout less aggressive and less responsive to stress.[11] However, the study mentions that plasma cortisol was not affected by dietary L-tryptophan. The drug LY354740 (also known as Eglumegad, an agonist of the metabotropic glutamate receptors 2 and 3) has been shown to interfere in the HPA axis, with chronic oral administration of this drug leading to markedly reduced baseline cortisol levels in bonnet macaques (Macaca radiata); acute infusion of LY354740 resulted in a marked diminution of yohimbine-induced stress response in those animals.[12]

Experimental studies have investigated many different types of stress, and their effects on the HPA axis in many different circumstances.[8] Stressors can be of many different types—in experimental studies in rats, a distinction is often made between "social stress" and "physical stress", but both types activate the HPA axis, though via different pathways.[9] Several monoamine neurotransmitters are important in regulating the HPA axis, especially dopamine, serotonin and norepinephrine (noradrenaline). There is evidence that an increase in oxytocin, resulting for instance from positive social interactions, acts to suppress the HPA axis and thereby counteracts stress, promoting positive health effects such as wound healing.[10]

The HPA axis is involved in the neurobiology of mood disorders and functional illnesses, including anxiety disorder, bipolar disorder, insomnia, posttraumatic stress disorder, borderline personality disorder, ADHD, major depressive disorder, burnout, chronic fatigue syndrome, fibromyalgia, irritable bowel syndrome, and alcoholism.[6] Antidepressants, which are routinely prescribed for many of these illnesses, serve to regulate HPA axis function.[7]

Stress and disease


[2] There is bi-directional communication and feedback between the HPA axis and immune system. A number of

Immune system

Glucocorticoids have many important functions, including modulation of stress reactions, but in excess they can be damaging. Atrophy of the hippocampus in humans and animals exposed to severe stress is believed to be caused by prolonged exposure to high concentrations of glucocorticoids. Deficiencies of the hippocampus may reduce the memory resources available to help a body formulate appropriate reactions to stress.

Increased production of cortisol during stress results in an increased availability of glucose in order to facilitate fighting or fleeing. As well as directly increasing glucose availability, cortisol also suppresses the highly demanding metabolic processes of the immune system, resulting in further availability of glucose.[2]

Anatomical connections between brain areas such as the amygdala, hippocampus, prefrontal cortex and hypothalamus facilitate activation of the HPA axis. Sensory information arriving at the lateral aspect of the amygdala is processed and conveyed to the central nucleus, which projects to several parts of the brain involved in responses to fear. At the hypothalamus, fear-signaling impulses activate both the sympathetic nervous system and the modulating systems of the HPA axis.

The HPA axis has a central role in regulating many homeostatic systems in the body, including the metabolic system, cardiovascular system, immune system, reproductive system and central nervous system. The HPA axis integrates physical and psychosocial influences in order to allow an organism to adapt effectively to its environment, use resources, and optimize survival.[2]

[5].burnout and [4]insomnia [3],chronic fatigue syndrome An abnormally flattened circadian cortisol cycle has been linked with [2]

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